Microsc. Microanal. Microstruct. 2, 281-291 (1991)
DOI: 10.1051/mmm:0199100202-3028100

Comparison of the simplex method with several other methods for background-fitting for electron energy-loss spectral quantification of biological materials

Wim De Bruijn¹, Diane Ketelaars¹, Edzard Gelsema² et Lianne Sorber<sup>1

1</sup>  AEM-unit, Clinical Pathology I, Erasmus University Rotterdam, Medical Faculty, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands
²  Dep of Medical Informaticas, Erasmus University Rotterdam, Medical Faculty, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands

Abstract
The application of the Simplex optimization procedure allows in functions of the form I(E) = A*E-r, A and r values to be calculated. The use of element-containing Bio-standards with a known externally determined concentration aids in getting reproducible results. Due to the reproducible spectra, fitting-procedures can be compared. The application of a three-dimensional Simplex optimization allows three parameters mutually to be compared. In this way the minimum length of the fitting zone (Γ) can be determined in order to find a constant value of Rx.

PACS
0705K - Data analysis: algorithms and implementation; data management.
0781 - Electron and ion spectrometers.
8280 - Chemical analysis and related physical methods of analysis.

Key words
EEL spectroscopy -- Optimization method -- Simplex method -- Biological materials


© EDP Sciences 1991