Microsc. Microanal. Microstruct.
Volume 2, Number 2-3, April / June 1991
|Page(s)||281 - 291|
Comparison of the simplex method with several other methods for background-fitting for electron energy-loss spectral quantification of biological materialsWim De Bruijn1, Diane Ketelaars1, Edzard Gelsema2 et Lianne Sorber1
1 AEM-unit, Clinical Pathology I, Erasmus University Rotterdam, Medical Faculty, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands
2 Dep of Medical Informaticas, Erasmus University Rotterdam, Medical Faculty, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands
The application of the Simplex optimization procedure allows in functions of the form I(E) = A*E-r, A and r values to be calculated. The use of element-containing Bio-standards with a known externally determined concentration aids in getting reproducible results. Due to the reproducible spectra, fitting-procedures can be compared. The application of a three-dimensional Simplex optimization allows three parameters mutually to be compared. In this way the minimum length of the fitting zone (Γ) can be determined in order to find a constant value of Rx.
0705K - Data analysis: algorithms and implementation; data management.
0781 - Electron and ion spectrometers.
8280 - Chemical analysis and related physical methods of analysis.
EEL spectroscopy -- Optimization method -- Simplex method -- Biological materials
© EDP Sciences 1991